Genotyping of single nucleotide polymorphisms by melting curve analysis using thin film semi-transparent heaters integrated in a lab-on-foil system.

Dna Melting Curve Analysis on Semi-transparent Thin Film Microheater on Flexible Lab-on-foil Substrate

This paper presents genotyping on a novel microheater concept using semi-transparent copper microheaters manufactured by roll-to-roll and lift-off on polyethylene napthalate (PEN) foil. Using a mesh structure, heater surfaces have been realized in one single metallization step with a manufacturing robustness higher than conventional meander structures. The thermal distribution of the meshes, ev...

Genotyping common SNP and a microsatellite sequence closely linked to waxy gene in rice by DNA based markers

The potential of different DNA based molecular markers was examined for the detection of single nucleotide polymorphism (SNP) in the waxy gene and a microsatellite (SSR) sequence closely linked to it in a collection of rice varieties. DNA was extracted from leaf samples of 68 different rice cultivars by the CTAB method and specific primers were designed for the amplification of waxy gene and SS...

Genotyping Parkinson disease-associated mitochondrial polymorphisms.

OBJECTIVE The purpose of this study was to establish a system for rapidly detecting single nucleotide polymorphisms (SNPs) in mitochondrial DNA (mtDNA) using hybridization probes and melting temperature (T(m)) analysis. This technology should prove useful for population-based studies on the interaction between genetic factors and environmental exposures and the risk of Parkinson disease (PD). ...

Author's response to reviews High-throughput genotyping of multiple single nucleotide polymorphisms closely positioned in the Title: ovine PRNP gene using linear fluorescent probes and melting curve analysis Authors:

Chemical-mediated Melting Curve Analysis for Genotyping of Single Nucleotide Polymorphisms

This report describes a new microfluidic solid-phase chemical-mediated melting curve analysis method for Single Nucleotide Polymorphism (SNP) genotyping. The method is based on allele-specific denaturation of DNA duplexes upon exposure to dynamic chemical gradient. DNA duplexes conjugated on beads are captured in a microfluidic gradient generator device. The device is designed with dams, keepin...